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Journal of Young Pharmacists Journal of Young Pharmacists
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Year : 2009  |  Volume : 1  |  Issue : 1  |  Page : 46-48

GC-MS analysis of propolis of Indian origin

1 Lovely Professional University, Phagwara, Jalandhar, India
2 Dept. of Pharmacognosy, Al-Ameen College of Pharmacy, Hosur road, Bangalore, India
3 IIHR (Institute of Horticulture Research), Hessaraghatta, Bangalore, India
4 St. John's Pharmacy College, Bangalore, India

Correspondence Address:
N Kumar
Lovely Professional University, Phagwara, Jalandhar
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DOI: 10.4103/0975-1483.51876

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More than 300 constituents have been identified in different propolis samples. This study investigated the composition of ethanolic extracts of propolis samples collected from the Gujarat zone. A gas chromatography-mass spectrometry was carried out on a Agilent GC-MS 5975 under electron impact ionization (70 eV). The chromatographic column for the analysis was done by the HP5MS capillary column (30 m x 0.25 mm internal diameter). The carrier gas used was helium at a flow rate of 1 ml/min. The oven temperature was 100C to 280C with a constant increase of 10C. Propolis samples were analyzed with the column held initially at 60C for 2 min and then increased to 230C with a 2C/ min heating ramp and then kept at 230C for 3 min. Finally, the temperature was increased to 280C with a 3C/min heating ramp. The injection was performed in split mode at 220C. Sample volumes of 1 μl were injected and analyzed by GC-MS. The following compounds were identified for the first time in the propolis sample: p-coumeric acid, Benzyl cinnamate, 4-pentanoic acid, and Ferulic acid. The main type of the compound identified was fatty acids derivatives. Similar results were found by Silici, et al . [5] for the turkey propolis.

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