PHARMACEUTICAL ANALYSIS
Year : 2009  |  Volume : 1  |  Issue : 3  |  Page : 264-269

A new RP-HPLC method for simultaneous estimation of nebivolol hydrochloride and hydrochlorthiazide in dosage forms


1 ADL Department, Wokhardt Ltd, Aurangabad, Maharashtra, India
2 Department of Pharmaceutical Analysis, B. R. Nahata College of Pharmacy, Mandsaur, India
3 Department of Pharmaceutics, B. R. Nahata College of Pharmacy, Mandsaur, India
4 Zydus Research Center, Ahmedabad, Gujrat, India
5 Department of Q.A, Lachoo College of Science and Technology, Shastri Nagar, Jodhpur, Rajasthan, India

Correspondence Address:
Y Gupta
ADL Department, Wokhardt Ltd, Aurangabad, Maharashtra
India
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DOI: 10.4103/0975-1483.57078

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A new RP-HPLC method has been developed for the simultaneous estimation of nebivolol hydrochloride and hydrochlorothiazide in pharmaceutical dosage forms, using ultra violet (UV) detector. Elution was carried out using a mobile phase consisting of acetonitrile and potassium dihydrogen phosphate buffer (pH 3.2 ± 0.1) in the ratio of 50:50 v/v and flow rate was set on 1.2 ml/min at 282 nm. The retention time for hydrochlorothiazide and nebivolol hydrochloride was found 3.57 and 6.66 mins, respectively. The method was found to be linear in the range of 8-32 mg/ml and 20-80 mg/ml for nebivolol hydrochloride and hydrochlorothiazide, respectively. In the linearity study, regression equation and coefficient of correlation for nebivolol hydrochloride and hydrochlorothiazide were found to be: y = 284761x + 215452, r = 0.9995; and y = 698395+105272, r = 0.9998, respectively.


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