|Year : 2010 | Volume
| Issue : 3 | Page : 273-279
Synthesis, characterization, and biological evaluation of benzimidazole derivatives as potential anxiolytics
DT Nannapaneni1, VSSS Gupta Atyam2, MI Reddy1, Sarva Ch Raidu2
1 Department of Pharmaceutical Chemistry, Mallareddy College of Pharmacy, Dhullapally Village, Hyderabad - 500 014, India
2 Department of Pharmacology, Joginpally B.R Pharmacy College, Yenkapally (P.O), Moinabad (Mandal), Ranga Reddy (Dist), Andhra Pradesh, India
|Date of Web Publication||27-Jul-2010|
D T Nannapaneni
Department of Pharmaceutical Chemistry, Mallareddy College of Pharmacy, Dhullapally Village, Hyderabad - 500 014
| Abstract|| |
The synthesized benzimidazoles compounds were prepared from the condensation reaction between o-Phenylenediamine and various carbonyl compounds, in the presence of ammonium chloride as a catalyst. Ammonium chloride is a commercial and environmentally benign catalyst. The yield of all benzimidazole derivatives was found to be in the range of 75 - 94%. The purity of the compounds was ascertained by melting point and TLC. The synthesized compounds were characterized by using IR, 1 H NMR, and MASS spectral data together with elemental analysis. The synthesized benzimidazole compounds were screened for acute and chronic anti-anxiety activity in Wistar rats by using an elevated plus maze model with standard Diazepam. The synthesized compounds Z B , Z E , Z F , Z G , and Z H showed potent anti-anxiety activity when compared to the standard Diazepam. The compound Z H exhibited a higher anti-anxiety activity when compared to other prepared benzimidazoles. The results were subjected to statistical analysis by using one-way ANOVA followed by the Tukey-Kramer test, to calculate the significance.
Keywords: Ammonium salts, anti-anxiety activity, benzimidazoles, carbonyl compounds, elevated plus maze model
|How to cite this article:|
Nannapaneni D T, Gupta Atyam V, Reddy M I, Raidu SC. Synthesis, characterization, and biological evaluation of benzimidazole derivatives as potential anxiolytics. J Young Pharmacists 2010;2:273-9
|How to cite this URL:|
Nannapaneni D T, Gupta Atyam V, Reddy M I, Raidu SC. Synthesis, characterization, and biological evaluation of benzimidazole derivatives as potential anxiolytics. J Young Pharmacists [serial online] 2010 [cited 2014 Apr 24];2:273-9. Available from: http://www.jyoungpharm.in/text.asp?2010/2/3/273/66809
| Introduction|| |
Benzimidazoles and its derivatives represent one of the most biologically active class of compounds, possessing a wide spectrum of activities and these are well-documented in literature. They show selective neuropeptides YY 1 receptor antagonists,  potent inhibitors of TIE-2 and VEGFER-2 tyrosine kinase receptors,  antitumor agents,  gamma-amino butyric acid (GABA) agonists, and 5-HT 3 antagonists.  Substituted benzimidazole derivatives have found commercial application in veterinarian medicine as anthelmintic agents and in diverse human therapeutic areas such as treatment of ulcers and antihistaminic.  Similarly, the general synthesis of benzimidazoles is by the condensation reaction of 1,2-phenylenediamine with carboxaldehydes, carboxylic acids, , or their derivatives , such as, chlorides, nitriles, and orthoesters, under strong acidic conditions, with high temperatures. Benzimidazoles have also been prepared on a solid phase to prove a combinatorial approach.  The most popular strategies for their synthesis utilize N-alkylation of an unsubstituted benzimidazoles.  Ammonium salts are inexpensive, commercially available reagents for few organic transformation reactions such as halogenation of aromatic compounds and synthesis of 3, 4-dihydropyrimidine-2(1H)-ones.  However, there are no reports of the use of ammonium salts as catalysts for the synthesis of benzimidazoles. In continuation, on the synthesis of heterocycles , and on the development of synthetic methodologies, ,, we herein report a facile method for the synthesis of benzimidazoles by the condensation of 1, 2-phenylenediamine with carbonyl compounds, in the presence of ammonium salts in very good yields. Anxiety is a psychological and physiological state characterized by cognitive, somatic, emotional, and behavioral components. These components combine to create an unpleasant feeling that is typically associated with uneasiness, fear or worry. Anxiety is a generalized mood or condition that occurs without an identifiable triggering stimulus. As such, it is distinguished from fear, which occurs in the presence of an observed threat. Additionally, fear is related to the specific behaviors of escape and avoidance, whereas, anxiety is the result of threats that are perceived to be uncontrollable or unavoidable. Some reports state that benzimidazoles possess anti-anxiety activity.  This observation prompted us to evaluate the synthesized benzimidazole derivatives for anti-anxiety activity
| Materials and Methods|| |
All the chemicals and reagents used were of analytical grade and were procured from NICE Chemicals. It is known that the reaction of o-phenylenediamine (OPDA) with carbonyl compounds, under strong acidic conditions, gives benzimidazoles, whereas, OPDA in the presence of β-ketoesters under neutral reflux conditions, gives benzodiazepin-2-ones, with the elimination of water and alcohol. Under acidic conditions, initially it forms ethyl β-2-amino aniline crotonate at room temperature and upon heating it gives 2-methyl-1H-benzo[d]imidazole instead of benzodiazepin-2-ones, with the elimination of ethyl acetate. Therefore, we have made an attempt to react OPDA with carbonyl compounds and b-ketoesters, with different ammonium salts to see the feasibility of the formation of compounds. To select favorable reaction conditions, we first examined the model reaction of 1, 2-phenylenediamine (1 mol) with benzaldehyde (1 mol) in the presence of NH 4 Br (1 mol), under solvent-free conditions, at room temperature. The reaction was monitored by thin-layer chromatography (TLC, eluent Hexane/ethyl acetate 30/70) and 2-phenyl-1H-benzo[d]imidazole obtained in 20% yield. Similarly, the reaction was conducted in different solvents such as CH3CN, MeOH, CHCl3, ether, and DMF; CHCl3 was found to be the most suitable solvent that gave benzimidazole with 40% yield. Next, we carried out the same reaction with different ammonium salts such as NH4F, NH4Cl, NH4NO3, (NH4)2CO3, and (NH4)2SO4 in the presence of CHCl3, at room temperature; among these, NH4Cl (4 mol) gave 2-phenyl-1H-benzo[d]imidazole with 94% yield in 4 hours [Figure 1], [Table 1]. The results, tabulated in [Table 2], indicate the formation of benzimidazoles.
The synthesized compounds were analyzed by NMR, Mass, and IR spectroscopy.1H NMR spectra were recorded on a Varian Gemini 200- and 300-MHz instrument in CDCl3 and DMSO-d6 using Tetramethylsilane (TMS) as an internal standard. The mass spectra were measured on a Liquid Chromatography / Mass Spectrometry (LCMS) Agilent mass spectrometer. The IR spectra were recorded on a Nicolet 740 Fourier transform infrared (FTIR) spectrometer. The melting points were measured using a Buchi-510 apparatus and were uncorrected.
Typical Experimental Procedure for the Synthesis of Benzimidazoles
Benzaldehyde (Y, 1 mmol) was added to a stirred solution of 1,2-phenylenediamine (X, 1 mmol) and NH4Cl (4 mmol) in CHCl3 (5 ml) for five minutes at room temperature. Stirring was continued for four hours. After completion of the reaction (TLC, eluent Hexane / ethyl acetate 30 / 70), the solvent was removed under reduced pressure and extracted with ethyl acetate (20 ml); the organic layer was washed with water (10 ml). Layers were separated and the organic layer was dried over sodium sulfate. The solvent was removed under reduced pressure and the crude product was subjected to column chromatography using petroleum ether = EtOAc (9:1), which gave 2-phenyl-1H-benzo[d]imidazole (Z A ) as a solid in 94% yield.
Solid; Molecular formula: C 13 H 10 N 2 , Yield-94%, m.p-296 o c;1H NMR: δ 6.06 (bs, 1H, NH), 6.82 (d,2H,aromatic), 6.98 (d,2H, aromatic), 7.06 (t,1H, aromatic),7.28 (m, 2H, aromatic), 7.52 (m, 2H, aromatic), IR (KBr): 3426(-NH), 3042(Ar-CH), 1742, 1631(-C = N) cm-1; Mass (LCMS): m/z 195 (M + + H).
Solid; Molecular formula:C 13 H 10 N 2 O, Yield - 78%, m.p-271 o c; 1H NMR: δ 6.06 (bs, 1H, NH), 6.82 (d,2H,aromatic), 6.98 (d,2H, aromatic), 7.21 (d,2H, aromatic),7.52 (d, 2H, aromatic), IR (KBr): 3379(-NH), 3211(-OH), 3078(-Ar-CH), 1461(C = N) cm-1; Mass (LCMS): m/z 211 (M + + H).
Solid; Molecular formula:C 13 H 9 N 3 O 2 , Yield -82%, m.p-208 o c; 1H NMR: δ 6.08 (bs, 1H, NH), 6.90 (d,2H,aromatic), 6.96 (d,2H, aromatic), 7.05 (t,1H, aromatic), 7.54 (d,1H, aromatic),8.12 (d, 1H, aromatic), 8.44 (s, 1H, aromatic), IR (KBr): 3211(-NH), 2984(Ar-CH), 1552(-NO 2 ), 1529(-C=N) cm-1; Mass (LCMS): m/z 240 (M + +H).
Solid; Molecular formula:C 14 H 12 N 2 O, Yield -85%, m.p-285 o c; 1H NMR: δ 3.70 (d,3H, OCH 3 ), 6.12 (bs, 1H, NH), 6.94 (d,2H,aromatic), 6.98(d,2H, aromatic), 7.20 (d,2H, aromatic), 7.58 (d,2H, aromatic), IR (KBr): 3294(-NH), 3103(Ar-CH), 1184 (-OCH 3 ), 1588(-C=N) cm-1; Mass (LCMS): m/z 225 (M + +H).
Solid; Molecular formula:C 13 H 12 N 2 , Yield -88%, m.p-278 o c; 1H NMR: δ 2.54 (d,3H,CH 3 ),6.06 (bs, 1H, NH), 6.84 (d,2H,aromatic), 6.96 (d,2H, aromatic), 7.18 (d,2H, aromatic),7.58 (d, 2H, aromatic), IR (KBr): 3346(-NH), 3024(Ar-CH), 2923(-CH 3 ), 1575(-C=N) cm-1; Mass (LCMS): m/z 209 (M + +H).
Solid; Molecular formula:C 13 H 9 N 3 O 3 , Yield -76%, m.p-237 o c; 1H NMR: δ 6.08 (bs, 1H, NH), 6.74 (d, 2H, aromatic), 6.84 (d, 2H, aromatic), 7.45 (d,1H, aromatic), 8.02 (d,1H, aromatic),8.32 (s, 1H, aromatic), IR (KBr): 3737(-OH),3432(-NH), 3103(Ar-CH), 1562(C=N), 1532(-NO 2 ) cm-1; Mass (LCMS): m/z 256 (M + +H).
Solid; Molecular formula:C 14 H 14 N 3 O 2 , Yield -86%, m.p-223 o c; 1H NMR: δ 2.56 (d,3H,CH 3 ),6.10 (bs, 1H, NH), 6.80 (d,2H,aromatic), 6.86 (d,2H, aromatic), 7.54 (d,1H, aromatic),8.08 (d, 2H, aromatic), 8.44 (s, 1H, aromatic), IR (KBr): 3402(-NH), 3054(Ar-CH), 1534(-C=N), 1524(-NO 2 ) cm-1; Mass (LCMS): m/z 254 (M + +H).
Solid; Molecular formula:C 14 H 12 N 2 O, Yield -82%, m.p-245 o c; 1H NMR: δ 2.56 (d,3H,CH 3 ), 6.48 (bs, 1H, NH), 6.78 (d,2H,aromatic), 6.92 (d,2H, aromatic), 7.48 (d,1H, aromatic), 8.10 (d,1H, aromatic), 8.44 (s,1H, aromatic), IR (KBr): 3455(-OH), 3274(-NH), 3212(Ar-CH), 2898(-CH 3 ), 1534(-C=N) cm-1; Mass (LCMS): m/z 225 (M + +H).
Wistar rats weighing 200 ± 25 g, of either sex, were procured at least two weeks prior to the study. The animals were housed in polycarbated cages under conditions of constant temperature (22 ± 2 o C) and humidity, under a 12-hour light / dark schedule, The animals were allowed free access to a standard diet and water, so they could acclimatize to the new environment.
Acute oral toxicity studies
The aim of this study is to determine the lethal dose. In this study the testing drug was administered in a single dose, using the oral route. The dose was increased in a graded manner. LD 50 in the acute toxicity test was observed at the dose of 400 mg/kg-bw. Therefore, one-tenth of the preceding dose (200 mg/kg-bw) was selected for the study, that is, 20 mg/kg-bw. This 20 mg/kg-bw testing dose did not have any impact on the normal locomotion of the animal, which was tested by administering the same dose to the animals, using the oral route. The locomator activity was assessed in an actophotometer.
In this activity, the elevated plus maze model was used. For this model, Wistar rats were divided into 10 groups of six animals each. Group-I (control) animals were administered the vehicle, Group-II, Group-III, Group-IV, Group-V, Group-VI, Group-VII, Group-VIII, and Group-IX animals were administered the benzimidazole derivatives of Z A - Z H, respectively, with a dose of 20 mg/kg-bw (p.o), and Group-X was administered diazepam 2 mg/kg-bw (p.o), for 1 day in case of acute study and for 10 days in case of chronic study. The number of entries and time spent in the open and closed arms of the elevated plus maze, using rats, was observed in acute and chronic studies for 1day and 10 days, respectively. Diazepam was used as a reference standard. The experiment was conducted in a sound attenuated room. In an acute study, the animals of all groups were treated with the respective drugs 30 minutes prior to the experiment. In a chronic study, animals of all groups were treated with the respective drugs for 10 days, and on the tenth day, treatment was given 30 minutes prior to the experiment. In both acute and chronic studies, each rat was placed in the center of the maze facing one of the enclosed arms, and during a ten-minute session the following parameters were noted; Number of entries into the open arm, number of entries into the closed arm, time spent in the open arm, time spent in the closed arm, and total number of entries into the open and closed arms. ,
The values were expressed as mean ± SEM for six animals. The results were subjected to statistical analysis by using one-way ANOVA followed by Tukey-Kramer test, to calculate the significant difference, if any, among the groups. P < 0.05 was considered as significant.
| Results|| |
The compounds (Z A - Z H ) were obtained by the reaction between aldehyde and o-phenylediamine in the presence of ammonium salts according to [Figure 1]. The synthesized compounds were confirmed by thin layer chromatography (TLC), Melting Point (mp), IR, 1 H NMR, and mass spectroscopy (MS) spectral analysis. The yields and melting points for all the synthesized compounds are listed in [Table 2]. The titled compounds were confirmed by IR spectral data showing characteristic bands at 1384 - 3200 cm -1 , indicating the presence of -NO 2 and -OH stretching; and sharp bands, ranging between 1680 - 1750 cm -1 , indicating the presence of C = N. Compounds Z A - Z H were confirmed by stretching at 3500 cm -1 , due to the presence of -NH. Compounds Z A - Z H were confirmed by 1 H NMR spectral analysis. The NMR proton peak at 6.00 - 6.18 ppm revealed the presence of -NH. Further appearance of the molecular ion peak at 225 (m + 1) and 209 (m + 1) confirmed the structure of Z D and Z E . The synthesized compounds Z B , Z E , Z F , Z G , and Z H were found to have potent anti-anxiety activity. Compound Z H exhibited more activity when compared to other prepared benzimidazoles.
In the acute and chronic studies of elevated plus maze models [Table 3] and [Table 4], the number of entries into the open and closed arms and the time spent in the open arm were increased; and time spent in the closed arm was decreased in synthetic preparation-treated animals when compared with the control animals, which was comparable with that of the reference standard, diazepam.
| Discussion|| |
Several researchers reported a synthesis of benzimidazole derivatives, but in our present study we synthesized the benzimidazole derivatives by using ammonium salts as catalysts, which were inexpensive and decreased the reaction time, with very good yields. This method could be easily practiced in laboratories within the stipulated time.
In the evaluation of anti-anxiety activity, the experimental model used in our study was an elevated plus maze. This was based on the assumption that unfamiliar, non-protective, and brightly lit environmental stress provoked inhibition of normal behavior. This normal behavioral inhibition was further augmented in the presence of fear or an anxiety-like state.  The elevated plus maze test was a well-established animal model for testing anxiolytic drugs. A known anxiolytic drug, diazepam, was used as the standard, which is one of the well-recognized anxiolytic drugs. In this model, the changes that occurred due to anxiety were, decreased time spent in the open arm, increased time spent in the closed arm, and decreased number of entries between the arms, than that which was observed in the control animals.
After treatment with the synthetic preparation, at a dose of 20 mg/kg-bw, the compounds Z B , Z E , Z F , Z G , Z H , and the standard, significantly increased the time spent in the open arm, decreased the time spent in the closed arm, and increased the number of entries between the arms, in the acute study [Table 3]. All these may be due to decreased fear, increased exploratory behavior, and the behavioral disinhibitory effect  of the standard (Diazepam) and synthetic preparation. Similar findings were observed in the chronic study also [Table 4]. This may be due to the same mechanism, as stated earlier in the acute study.
| Conclusion|| |
The present study describes a simple, inexpensive, and easy method for synthesis of benzimidazole derivatives in a stipulated time, without using any drastic conditions. The yield of all benzimidazole derivatives were found to be in the range of 75 - 94%. The purity of the compounds were ascertained by a melting point and TLC. The assigned structure was further established by IR, 1 HNMR, and MS spectral studies.
The acute and chronic studies for anti-anxiety activity of the synthesized compounds were screened using elevated plus maze method in Wistar rats. Diazepam was used as the reference drug. In the prepared benzimidazole derivatives, it seemed that the compounds Z B [4-(1H-benzimidazol-2-yl) phenol], Z E [2-(4-methylphenyl)-1H-benzimidazole], Z F [4-(6-nitro-1H-benzimidazol-2-yl) phenol], Z G [2-(4-methylphenyl)-6-nitro-1H-benzimidazole], and Z H [4-(6-methyl-1H-benzimidazol-2-yl) phenol] showed potent activity when compared to the standard drug diazepam. The compound Z H [4-(6-methyl-1H-benzimidazol-2-yl) phenol] exhibited the highest anti-anxiety activity when compared to the other prepared benzimidazole compounds.
From the present study, it can be concluded that the benzimidazole derivatives can potentially be developed into useful anti-anxiety agents, which can prompt future researchers to synthesize a series of benzimidazole derivatives containing a wide variety of substituent's, with the aim of producing a novel heterocyclic system, with enhanced activity.
| References|| |
|1.||Zarrinmayeh H, Nunes AM, Ornstein PL, Zimmerman DM, Arnold MB, Schober DA, et al. Synthesis and evaluation of a series of novel 2-[(4-chlorophenoxy)methyl]benzimidazoles as selective neuropeptide Y Y1 receptor antagonists. J Med Chem 1998;41:2709-19. [PUBMED] [FULLTEXT] |
|2.||Hasegawa M, Nishigaki N, Washio Y, Kano K, Harris PA, Sato H, et al. Discovery of novel benzimidazoles as potent inhibitors of TIE-2 and VEGFR-2 tyrosine kinase receptors. J Med Chem 2007;50:4453-70. [PUBMED] [FULLTEXT] |
|3.||Hranjec M, Kralj M, Piantanida I, Sedic M, Suman L, Pavelic K, et al. Novel cyano- and amidino-substituted derivatives of styryl-2-benzimidazoles and benzimidazo[1,2-a]quinolines. Synthesis, photochemical synthesis, DNA binding, and antitumor evaluation, part 3. J Med Chem 2007;50:5696-711. |
|4.||Falco J, Pique M, Gonzalez M, Buira I, Mendez E, Terencio J, et al. Synthesis, pharmacology, and molecular modeling of N-substituted 2-phenyl-indoles and benzimidazoles as potent GABA(A) agonists. Eur J Med Chem 2006;41:985-90. |
|5.||Spasov AA, Yozhitsa IN, Bugaeva LI, Anisimova VA. Rearrangement strategy for the syntheses of 2-amino anilines. Pharm Chem J 1999;33:232-43. |
|6.||Phillips MA. The formation of 2-substituted benzimidazoles. J Chem Soc 1928;JR9280002393:2393. |
|7.||Grimmet MR, Katritzky AR, Rees CW. Hetero cyclic chemistry. Vol. 5. Oxford, UK: 1984. p. 457. |
|8.||Czarny A, Wilson WD, Boykin DW. Synthesis of mono cationic and dicationic analogs of Hoechst 33258. J Heterocycl Chem 1996;33:1393. |
|9.||Tidwell RR, Geratz JD, Dann O, Volz G, Zeh D, Loewe H. Diarylamidine derivatives with one or both of the aryl moieties consisting of an indole or indole-like ring. Inhibitors of arginine-specific esteroproteases. J Med Chem 1978;21:613-23 . [PUBMED] |
|10.||Wu Z, Rea P, Wickam G. Tetrahedron Lett. 2000;41:9871-4, and the reference cited therein; Mazurov A. Bioorg Med Chem Lett 2000;10:67-70. |
|11.||Kim BH, Han R, Kim JS, Jun YM, Baik W, Lee BM. Simple and mild procedures for synthesis ofbenzimidazoles using heterogeneous catalyst systems. Heterocycles 2004;62:41-54. |
|12.||Gangadasu B, Narender P, Ramesh C, China Raju B, Rao VJ. Facile and selective synthesis of chloronicotin aldehydes by the vilmeier reaction. Tetrahedron 2006;62:8398. |
|13.||China Raju B, Neelakantan P, Bhalerao UT. Quinone methide initiated cyclization reaction: Synthesis of 4-aryl-1,2,3,4-tetrahydroisoquinolines. Tetrahedron Lett 2004;45:7487. |
|14.||Gangadasu B, China Raju B, Rao VJ. A simple and convenient preparation of 2-chloro-5-methylpyridine-3-carbaldehydeimines. Heterocycl Comm 2002; 8: 243. |
|15.||Narender P, Gangadasu B, Ramesh C, China Raju B, Rao VJ. Facile and selective synthesis of chloromethylpyridines and chloropyridines using diphosgene-triphosgene. Synth Commun 2004;34:1097. |
|16.||Babu KS, China Raju B, Srinivas PV, Rao JM. Highly efficient and chemo selective cleavage of prenyl ethers using ZrCl4-NaBH4. Tetrahedron Lett 2003;44:2525. |
|17.||Babu KS, China Raju B, Srinivas PV, Rao AS, Kumar SP, Rao JM. A simple, effective, and highly selective cleavage of 3-methylbut-2-enyl (prenyl) ethers using p-toluenesulfonic acid. Chem Lett 2003;32:704. |
|18.||Maryanoff BE. Potential anxiolytic agents. Pyrido [1, 2-a] benzimidazoles: A new structural class of ligands for the benzodiazepine binding site on GABA-A receptors. J Med Chem 1995;38:16-20. |
|19.||Latif N, Mishriky N, Assad FM, Meguid SB. b-Nitrostyrenes with o-phenylenediamine: A new route of the synthesis of 2-substituted benzimida-zoles. Indian J Chem 1982;21B:872. |
|20.||Bougrin K, Soufiaoui M. Nouvelle voie de synthese arylimidazoles sous irradiation microondes enmilieusec.Arylimindazoles prepared on a microwave irradiation in domestic ovens. Tetrahedron Lett 1995;36:3683. |
|21.||Matsushita H, Lee SH, Joung M, Clapham B, Janda KD. Smart cleavage reactions: The synthesis of benzimidazoles and benzothiazoles from polymer-bound esters. Tetrahedron Lett 2004;45:313. |
|22.||Kim JS, Sun Q, Gatto BG, Yu C, Liu A, Liu LF, et al. Structure-activity relationships of benzimidazoles and related heterocycles as topoisomerase I poisons. Bioorg Med Chem 1996;4:621-30. |
|23.||Hogg SA. Review of the validity and variability of the elevated plus maze as an animal model of anxiety. Pharmacol Biochem Behav 1996;54:21-30. |
|24.||Gopal Krishna HN, Sangha RB, Miara N, Pai MR. Antianxiety activity of NR-ANX-C, a polyherbal preparation in rats. Indian J Ethno Pharmacol 2006;38:330-5. |
[Table 1], [Table 2], [Table 3], [Table 4]
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