Year : 2009  |  Volume : 1  |  Issue : 3  |  Page : 264-269

A new RP-HPLC method for simultaneous estimation of nebivolol hydrochloride and hydrochlorthiazide in dosage forms

1 ADL Department, Wokhardt Ltd, Aurangabad, Maharashtra, India
2 Department of Pharmaceutical Analysis, B. R. Nahata College of Pharmacy, Mandsaur, India
3 Department of Pharmaceutics, B. R. Nahata College of Pharmacy, Mandsaur, India
4 Zydus Research Center, Ahmedabad, Gujrat, India
5 Department of Q.A, Lachoo College of Science and Technology, Shastri Nagar, Jodhpur, Rajasthan, India

Correspondence Address:
Y Gupta
ADL Department, Wokhardt Ltd, Aurangabad, Maharashtra
Login to access the Email id

DOI: 10.4103/0975-1483.57078

Get Permissions

A new RP-HPLC method has been developed for the simultaneous estimation of nebivolol hydrochloride and hydrochlorothiazide in pharmaceutical dosage forms, using ultra violet (UV) detector. Elution was carried out using a mobile phase consisting of acetonitrile and potassium dihydrogen phosphate buffer (pH 3.2 ± 0.1) in the ratio of 50:50 v/v and flow rate was set on 1.2 ml/min at 282 nm. The retention time for hydrochlorothiazide and nebivolol hydrochloride was found 3.57 and 6.66 mins, respectively. The method was found to be linear in the range of 8-32 mg/ml and 20-80 mg/ml for nebivolol hydrochloride and hydrochlorothiazide, respectively. In the linearity study, regression equation and coefficient of correlation for nebivolol hydrochloride and hydrochlorothiazide were found to be: y = 284761x + 215452, r = 0.9995; and y = 698395+105272, r = 0.9998, respectively.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded312    
    Comments [Add]    
    Cited by others 1    

Recommend this journal